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NEPETALACTONE Communications FDA White Oak Labs Finds 6X to 470X DNA Contamination in MRNA Vaccine …MRNA疫苗中发现6X至470倍的DNA污染


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2025 Jan 4, 1:35pm   24 views  1 comment

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NEPETALACTONE Communications FDA White Oak Labs Finds 6X to 470X DNA Contamination in MRNA Vaccine …MRNA疫苗中发现6X至470倍的DNA污染

Unacceptable Jessica cross-posted a post from Maria Gutschi

Jessica Rose
Jan 4 · Unacceptable Jessica
Please read

https://open.substack.com/pub/mariagutschi/p/the-analysis-of-residual-dna-in-a

The analysis of residual DNA in a High School Science Journal
A few observations
WASHED UP PHARMACIST
JAN 4

READ IN APP

MaryAnne Demasi reports on an extra-ordinary paper.

Maryanne Demasi, reports
EXCLUSIVE: FDA lab uncovers excess DNA contamination in COVID-19 vaccines
An explosive new study conducted within the U.S. Food and Drug Administration’s (FDA) own laboratory has revealed excessively high levels of DNA contamination in Pfizer’s mRNA COVID-19 vaccine…
Read more
2 days ago · 294 likes · 19 comments · Maryanne Demasi, PhD
This study was performed in a BSL-1 lab at the FDA White Oak Campus, by high school students under supervision of FDA scientists. This was published December 28th, and however Maryanne found it, or was tipped off to it, we thank her and her possible accomplices. Others have written on the pros an cons of this paper much better than I can. I just want to make a few observations that are bothering me for discussion.

Read Kevin’s great review. Congratulations to these high school students.

Nepetalactone Newsletter
FDA White Oak Lab Finds 6X to 470X DNA contamination in mRNA vaccines
Fellow Anandamigo Maryanne Demasi, PhD alerted me to a publication that came out in the Journal of High School Science that found 6-470 times the 10ng DNA FDA limit in Pfizer mRNA vaccines (Wang et al…
Read more
a day ago · 197 likes · 49 comments · Anandamide
OK, here are some interesting observations of my own.

1. They made their own mRNA vaccine

So a common plasmid (vector) was used containing the XBB.1.5 sequence. The pcDNA3.1(+) vector is a mammalian expression vector with the CMV promoter (made by ThermoFischer or Invitrogen?) The MCS is in the forward (+) orientation. I don’t know what that means. Anyone?

It also has an SV40 promoter and SV40 ori, and an AmpR promoter and gene (instead of kanamycin). I THINK this is the right plasmid vector. If I have it wrong, please correct me in the comments. Did they grow this in E coli cells? I’m very confused.

Then they made their own vaccine using a kit. I am wondering where the IVT step went. This is the “kit” for Cayman Chemical

And here is the description. So you don’t need a T-mixer or a herringbone mixer or any specialized equipment. Who knew it was so easy to make mRNA/LNP products?

So this is using the Moderna ionizable and pegylated lipid in this kit. You can even hand mix them!! After that you better filter out the big LNP particles though because you won’t get a homogenous mixture.

Don’t try this at home, folks. They helpfully add, that it is not for human use. Well, neither was SM-102 when it was approved.

Still, where is the IVT step to make the oligonucteotide, or mRNA. They don’t mention it.

2. They got a hold of some COVID-19 “biosimilar” mRNA vaccines (WTH!!)

OK, to pharmacists and the industry, “biosimilar” means an mRNA covid vaccine NOT MADE BY PFIZER or MODERNA. Here is a quick and dirty explanation. For these vaccines, given the different manufacturing process, Process 2 Pfizer is a biosimilar to Process 1, ie another drug.

So what is this ‘biosimilar’ vaccine? Does the NIH have some frozen Process 1 vials lying around? Are these the early Process 2 vials approved under EUA (ie before Aug 2021 for Pfizer and Jan 2022 for Moderna)? Or are these trial vaccines made by another manufacturer or university?

BEI resources is an interesting website. You can order all kinds of reagents, pool blood with antibodies, anything you need to do infectious disease resources. Including mRNA vaccines. Weird. I couldn’t find the specific catalogue number on this site, but maybe I needed to register and be vetted. If anyone has used reagents etc from BEI resources, can they look to see if NR-59604 and NR-59605. Well none to be had, but they add helpfully, that MADE TO ORDER products are excluded. So maybe they were made to order? After all, there are kits to make your OWN mRNA products. Who knew?

3. Extraction of the DNA from the mRNA vaccine

They use a DNA extraction kit to extract the residual DNA in the vaccines.

Kevin talks about what this kit does in his substack article for some of the problems he identified with this prep kit. Though is DOES have RNase A as part of the kit to get rid of any interfering RNA.

One problem I have however is,

WHAT DID THEY DO TO CRACK OPEN THE LNPS?

Did they do this step? Or are they just measuring the DNA outside of the LNPs in the sucrose/Tris formulation? I mean, this has been one of the hardest nuts to crack. Use Triton-X (a surfactant) like Kammerer used, or a boil prep like McKernan and Speicher used. Did they do anything?

4. How many total mRNA vaccines did they test?

OK, so for determining replication competent DNA the used the in-house mRNA vaccine they made, plus the 2 “biosimilars” so 3 altogether.

Then after establishing this method they tested it in 2 commercial Pfizer lots.

Here they only report the PAA number but their picture shows the lot numbers.

If you do a search for PAA184098 you find this image (as did Geoff Pain)

This is the monovalent Pfizer vaccine with the trade name Comirnaty. The one above this is an older Pfizer vaccine WITHOUT the trade name…… could this be what they call a “biosimilar?”

If you do a search for the top vial, PAA194854 you get this, found on a Canadian site but I cannot find it in the Canadian National Vaccine Catalogue. It is the BA/4/BA.5 bivalent. A lot of people get the PAA and the Lot numbers mixed up in real life. It is not as obvious as you would think.

It looks like the students got the bivalent and movalent mixed up. It is VERY EASY to do so, and this has led to many administration errors in VAERS.

So a total of 5 vaccines. The picture of panel A of the thawing vaccines are just pictures of these 2 Pfizer lots. Wonder where they got them from? Most pharmacies would have gotten rid of these long ago so I think these were obtained from the federal stockpile. In Canada, all vaccines go to a central sorting station are logged then sent out to the provinces depending of what the province orders.

5. The FDA scientists who supervised this research won an award on a new COVID-19 vaccine

The scientists who supervised this research should be commended. They are

S Liu
P Selvaraj
T. Wang (possibly the father on Tyler Wang, one of the students?)
They won the 2023 US FDA Laboratory award for:

Here is the paper

So what are these FDA researchers doing developing a LAV??? Which wins an award? Does the FDA think that mRNA vaccines are a failure? What is going on?

6. Siguna Mueller, Ph.D., Ph.D. has a very good take, imho

SUMMARY

This is a strange paper, imho. I agree with Siguna. THe conclusions can go either way, since they did not find replication competent DNA in the 2 Pfizer vaccine lots they studies, despite finding high total DNA levels via fluoroscopy.
I would like to know how they did the IVT for their home-made mRNA
And how they cracked open the LNPs, if that was done at all.
I am not sure this paper can be used to “get” the FDA, though the FDA supervisors are working on a TOTALLY different kind of vaccine.
Thoughts anyone?

Thanks for reading, and pray the rosary. This year will be crazy 不可接受的Jessica jessica jessica jessica jessica上涨1月4·在高中科学期刊中读取了对剩余DNA的分析,一些观察在App Maryanne Demas上阅读了药剂师1月4日阅读 Maryanne Demasi,报告是独家:FDA实验室在Covid-19疫苗中揭示过量的DNA污染,在美国食品和药物管理局(FDA)自己的实验室内进行的爆炸性新的研究表明,患有过高的DNA水平 这是12月28日出版的,然而玛丽安妮发现它,或者被拒绝了,我们感谢她和她的同罪。 其他人已经比我所能的好处写在这些论文中。 我只想造成一些困扰我讨论的观察。 阅读Kevin的伟大评论。 祝贺这些高中生。 NEPETALACTONE通讯FDA白橡木实验室在MRNA疫苗中发现6X至470倍的DNA污染,博士博士·米兰·马里安尼亚德拉斯省,博士提醒我发表于中国高中科学杂志的出版物6 我想知道IVT步骤的位置。 这是Cayman Chemical的“套件”,这里是描述。 因此,您不需要T型混频器或蜂鸣器搅拌机或任何专用设备。 谁知道MRNA / LNP产品很容易? 因此,这是在该试剂盒中使用现代可电离和聚乙二醇化的脂质。 你甚至可以混合它们! 之后,您可以更好地过滤出大型LNP粒子,因为您不会获得均匀混合物。 不要在家里试试这个。 他们有帮助地添加,它不适用于人类使用。 好吧,它批准后也不是SM-102。 仍然,在哪里制备寡核苷酸或mRNA的IVT步骤。 他们没有提到它。 2.他们持有一些Covid-19“生物仿制物”mRNA疫苗(WTH !!)OK,给药剂师和行业,“BioSimilar”是指辉瑞或现代的MRNA Covid疫苗。 这是一个快速而肮脏的解释。 对于这些疫苗,鉴于不同的制造过程,方法2辉瑞是一种生物仿制方法,即另一种药物。 那么这个'生物仿佛'疫苗是什么? NIH是否有一些冻结的过程1个小瓶躺在? 这些早期的过程2个小瓶批准在EUA批准(即在2021年8月为辉瑞公司之前的辉瑞和1月2022年)? 或者是另一种制造商或大学制造的这些试验疫苗? Bei Resources是一个有趣的网站。 您可以订购各种试剂,用抗体池血液,您需要做传染病资源的任何东西。 包括mRNA疫苗。 奇怪。 我找不到这个网站上的特定目录号码,但也许我需要注册并被审查。 如果有人使用了来自BEI资源的试剂等,他们可以看出NR-59604和NR-59605。 嗯,只有它们,但他们有用,以便排除订购产品。 也许他们是为了订购? 毕竟,有套件可以制作自己的mRNA产品。 谁知道? 3.从mRNA疫苗提取DNA,它们使用DNA提取试剂盒提取疫苗中的残留DNA。 Kevin谈到这个套件在他的家用文章中为他识别的一些问题讨论了他用这种预备套件确定的一些问题。 虽然确实具有RNase A作为套件的一部分,以摆脱任何干扰RNA。 然而,我的一个问题是,他们做了什么来破解LNP? 他们做了这一步吗? 或者他们只是测量蔗糖/三种制剂中LNP外的DNA? 我的意思是,这是最难破裂的坚果之一。 使用类似Kammerer的Triton-X(表面活性剂),或者煮沸准备,如麦克奈南和所使用的扑克。 他们做了什么吗? 4.他们测试了多少例mRNA疫苗? 好的,所以用于确定复制态度DNA,使用它们所做的内部mRNA疫苗,加上2“生物仿制物”所以3。 然后在建立这种方法后,他们在2个商业辉瑞公司中测试了它。 在这里,他们只报告PAA号码,但他们的照片显示了批号。 如果您进行搜索PAA184098,您将找到此图片(毕业疼痛)这是单价辉瑞疫苗,具有商品名的可口名称。 上面的那个是没有商品名的较旧的辉瑞疫苗......这可能是他们称之为“生物仿制物”的疫苗 如果您进行搜索顶级小瓶,PAA194854您可以在加拿大网站上找到它,但我无法在加拿大国家疫苗目录中找到它。 它是Ba / 4 / Ba.5二价。 很多人都会获得真实生活中的PAA和批次。 它不像你的想法那么明显。 看起来学生们得到了二价和移动混合。 这很容易这样做,这导致了仇恨中的许多管理错误。 所以共有5个疫苗。 解冻疫苗的面板A的图片只是这2个辉瑞批次的图片。 想知道他们从哪里得到它们? 大多数药店都会让这些很久以前摆脱,所以我认为这些是从联邦储存中获得的。 在加拿大,所有疫苗都将被记录到中央分拣站,然后根据省份订单发送到省份。 5.监督这项研究的FDA科学家赢得了一个新的Covid-19疫苗奖,这应该是监督这项研究的科学家。 他们是刘p selvaraj t. wang(可能是泰勒王,学生之一的父亲?)他们赢得了2023年美国FDA实验室奖:这是本文,这些FDA研究人员正在开发一个厕所??? 哪个奖项? FDA是否认为mRNA疫苗是失败的? 发生了什么? 6. Siguna Mueller,Ph.D.,Ph.D. 有一个非常好的,恕我直言摘要这是一个奇怪的论文,imho。 我同意Siguna。 尽管他们在2辉瑞疫苗批量疫苗批量中没有找到复制态度的DNA,但尽管通过荧光检查发现高总DNA水平,因此得出的结论可以进行任何一种方式。 我想知道他们是如何为自己的家庭制造的mRNA和它们如何破解LNPS的IVT,如果这完全完成了。 我不确定本文可用于“获得”FDA,尽管FDA主管正在进行完全不同的疫苗。 想到任何人? 谢谢你的阅读,祈祷念珠。 今年会疯了

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