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NEW STUDY:
Resveratrol and Copper Trigger System-Level
Collapse of Human
Glioblastoma
Aggressiveness in
Just 12 Days
In one of the deadliest human cancers, cheap nutraceuticals produced coordinated suppression of tumor proliferation, cancer hallmarks, immune checkpoints, stemness, and activated intrinsic apoptosis.
NICOLAS HULSCHER, MPH

对癌症的新研究：

白藜芦醇和铜在12天内触发人类胶质母细胞瘤的系统性崩溃，减缓其侵袭性

在这种致命性极高的人类癌症中，廉价的营养保健品协调性地抑制了肿瘤增殖、癌症标志物、免疫检查点、干性特征以及激活的内源性凋亡。

尼古拉斯·胡尔舍（Nicolas Hulscher），公共卫生硕士（MPH）

https://open.substack.com/pub/petermcculloughmd/p/new-study-resveratrol-and-copper

作者：Nicolas Hulscher，MPH

膠質母細胞瘤（GBM）仍然是最具有攻擊性和致命性的人類癌症之一，儘管有手術、放療和化療，其生存期中位數約為15個月。 在《BJC報告》新發表的一篇題為《白藜蘆醇和銅的預氧化組合後膠質母細胞瘤惡性表型衰減》的論文中，研究人員發現了一種簡短的、無毒的口服干預，可以同時抑制腫瘤增殖、癌症特徵、免疫檢查點和幹細胞——同時啟動內在腫瘤細胞死亡。

在一項小型但仔細控制的術前「視窗」研究中，人類膠質母細胞瘤患者每天四次服用白藜蘆醇（5.6毫克）和銅（560納克），平均在腫瘤切除前約12天。 然後將腫瘤組織與未經治療的對照組進行比較。

結果揭示了惡性表型的系統級衰減：幾乎根除促進腫瘤的無細胞染色質顆粒（cfChPs）——伴隨著腫瘤增殖（Ki-67）減少約31%，抑制九種癌症特徵和癌症幹細胞，同時降低六個免疫檢查點，並啟動內在細胞凋亡，所有這些都在約12天內。

這不是一個微不足道的訊號。 這是已知最致命的癌症之一的協調、系統層面的生物轉變：

by Nicolas Hulscher, MPH
Glioblastoma (GBM) remains one of the most aggressive and lethal human cancers, with a median survival of roughly 15 months despite surgery, radiation, and chemotherapy. In a newly published paper in BJC Reports titled, Attenuation of malignant phenotype of glioblastoma following a short course of the pro-oxidant combination of Resveratrol and Copper, researchers found a short, non-toxic oral intervention that simultaneously suppresses tumor proliferation, cancer hallmarks, immune checkpoints, and stemness — while activating intrinsic tumor cell death.
In a small but carefully controlled pre-surgical “window” study, human glioblastoma patients received resveratrol (5.6 mg) plus copper (560 ng) four times daily for an average of just ~12 days before tumor resection. Tumor tissue was then compared with untreated controls.
The results reveal a system-level attenuation of malignant phenotype: near-eradication of tumor-promoting cell-free chromatin particles (cfChPs)—accompanied by a ~31% reduction in tumor proliferation (Ki-67), suppression of nine cancer hallmarks and cancer stemness, simultaneous down-regulation of six immune checkpoints, and activation of intrinsic apoptosis, all within ~12 days.
This was not a marginal signal. It was a coordinated, system‑level biological shift in one of the deadliest cancers known:

Near‑Elimination of Tumor‑Promoting Cell‑Free Chromatin
Using confocal microscopy, investigators showed that glioblastoma tumors are saturated with extracellular DNA–histone complexes (cfChPs) — debris released from dying cancer cells that can enter neighboring cells and drive DNA damage, inflammation, immune evasion, and malignancy.
After short‑course resveratrol–copper treatment, these cfChPs were virtually absent from the tumor microenvironment. Quantitative analysis showed a highly significant reduction in extranuclear chromatin signal, indicating near‑eradication of this upstream oncogenic driver.

Large Reduction in Tumor Proliferation (Ki‑67)
Ki‑67 is a gold‑standard marker of how aggressively a tumor is dividing.

This represents a ~31% reduction in actively dividing tumor cells after less than two weeks of treatment. A shift of this magnitude suggests a real down‑staging of biological aggressiveness, even in the absence of visible histologic remodeling.

Simultaneous Suppression of 9 Hallmarks of Cancer
The authors examined 15 biomarkers spanning nine canonical hallmarks of cancer (genomic instability, inflammation, angiogenesis, invasion, metabolic reprogramming, etc.).

肿瘤促进性细胞外无细胞染色质的近乎消除

研究人员使用共聚焦显微镜显示，胶质母细胞瘤肿瘤中充满了细胞外DNA-组蛋白复合物（cfChPs）——这些是由死亡癌细胞释放的碎片，能够进入邻近的细胞并引发DNA损伤、炎症、免疫逃逸和恶性转化。

经过短期白藜芦醇-铜联合治疗后，这些cfChPs几乎完全从肿瘤微环境中消失。定量分析显示，细胞核外染色质信号显著减少，表明这种上游致癌因子的几乎根除。

腫瘤擴散大幅減少（Ki-67）

Ki-67是腫瘤分裂程度的黃金標準標誌。

這代表在治療不到兩週後，積極分裂的腫瘤細胞減少了約31%。 即使沒有可見的組織學重塑，這種幅度的轉變也表明了生物攻擊性的真正下降階段。

同時抑制癌症的9個特徵

作者檢查了15個生物標誌物，涵蓋癌症的九個規範特徵（基因組不穩定、炎症、血管生成、浸潤、代謝重新寫程式等）。

這種干預產生了廣泛、協調的惡性行為抑制。

This intervention produced broad, coordinated suppression of malignant behavior.

All were significantly down‑regulated in treated tumors (p < 0.0001 overall). Importantly, five of these checkpoints were shown to be expressed on tumor‑infiltrating lymphocytes, confirming biological relevance.

在治療的腫瘤中，所有腫瘤都明顯下調（p < 0.0001總體）。 重要的是，其中五個檢查點在腫瘤浸潤淋巴細胞上表達，證實了生物學相關性。